Gnotobiotic mouse technology : an illustrated guide /

Main Authors: Vowles, Chriss J., 1983, (Author), Anderson, Natalie E., 1986- (Author), Eaton, Kathryn A., (Author)
Format: Taylor Francis eBooks
Language:English
Published: Boca Raton : Taylor & Francis, [2016]
Subjects:
Online Access:Click here to view.
Table of Contents:
  • 1. Brief history of germ-free life
  • 2. Overview of gnotobiotic technology
  • 3. Equipment and terminology
  • 4. Personal protective equipment (PPE)
  • 5. Sterilants and sterilization
  • 6. Isolator setup
  • 7. Port entry and exit
  • 8. Sterilizing food and supplies
  • 9. Supply cylinder entry
  • 10. Isolator maintenance
  • 11. Aseptic mouse transfer
  • 12. Working with germ-free or gnotobiotic mice in the class II biosafety cabinet
  • 13. Shipping mice
  • 14. Rederivation
  • 15. Microbiological testing
  • 16. Genetic testing
  • 17. Facility setup and management.